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Monitoring oil biodegraders

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Monitoring dynamics of microbial community

Usually a combination of the following techniques is used in each study.

Culture dependent

Culture dependent techniques deal with living organisms and useful isolates can be obtained and subsequently studied. Many bacterial strains have been isolated from coastal and oceanic environments; these bacteria, including the genera Pseudomonas, Vibrio and Flavobacterium, have been considered to be representative of marine bacteria.

The following techniques are most commonly used:

Culture independent

It has been recognized that surveys of microbial diversity without cultivation are necessary as most of the microscopically detected microbial cells in seawater cannot be cultivated in conventional media.

The culture independent approaches exemplified by the construction of 16S rRNA gene (rDNA) clone libraries, fluorescence in situ hybridization (FISH) analysis with rRNA-targeted oligonucleotide probes, and denaturing gradient gel electrophoresis (DGGE) of PCR-amplified rDNA have thus been introduced in microbial ecology and have revealed an unexpectedly large diversity of marine bacteria.

Possible reasons for "unculturability" of many bacteria:

Bacteria retrieved by culture-dependent methods are generally quite different from those identified by the culture-independent rRNA approaches. The rDNA sequences of bacteria obtained by culture-dependent methods rarely appear in the rDNA library.

Culture independent techniques do not introduce bias from culture media, isolates are not obtained. The following techniques are most commonly used:

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References

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